以下为句子列表:
英文: This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in E. coli, and summarize the most efficient ways to refold recombinant proteins.
中文: 摘要描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性,综述了国内外变性、复性的有效方法。
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英文: The results indicate that cultivate on the table, the MT3-MMP expression amount is high when cells were induced by adding 0.5 mM IPTG for four hours when the cell density reached an A600 of 0.3-0.4. The inclusion bodies were purified through washing.
中文: 实验表明,采用摇床上培养时,MT3-MMP表达的最佳实验条件为:开始诱导时的菌体密度OD600=0.3—0.4; 诱导时间为4小时,IPTG浓度为0.5mM时,目的蛋白表达量较高。
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英文: The inclusion bodies of the pThr-2 produced in the E. coli culture achieved 110 mg/L, accounting for 21.7% of the total cell proteins.
中文: 最终在优化的培养条件下,重组凝血酶原-2包涵体的产量为110 mg/L发酵液,占菌体总蛋白的21. 7%。
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英文: Cloning and inclusion bodies expression of the gene of earthworm fibrin olytic enzyme
中文: 蚯蚓溶栓酶基因的克隆及非融合性表达
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英文: Comparison of Three Renaturation for the Inclusion Bodies of the Recombinant Chitinase C Producing Engineering Bacteria
中文: 重组产几丁质酶C工程菌包涵体的复性
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