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To establish a model for high throughput screening the agonist of GLP-1 receptor, the GLP-1 receptor gene was inserted into pCDNA3.1 carrier to establish GLP-1 R plasmid.
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本实验建立了GLP-1受体基因质粒和报告基因质粒,并将它们共同转染到CHO细胞、SHSY5Y细胞和HEK293细胞中,通过对EC50的测定,发现CHO细胞更适合作GLP-1R激动剂的高通量筛选。 |
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To establish an up-to-dete experimental study system for modern systemic immunology
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构建现代系统免疫学新的实验研究体系 |
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To explore clinical features and therapeutic evaluation of childhood anaplastic large cell non-Hodgkin's lym-phoma (CALCL), all clinical data were summarized and analyzed in 4 children with CALCL.
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为探讨儿童间变型大细胞非霍奇金淋巴瘤(CALCL)临床特点、方案选择及疗效分析,对4例儿童间变型大细胞性非霍奇金淋巴瘤患儿进行临床诊断、免疫分型、治疗、随访及疗效总结。 |
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To explore whether SEMA6A, B56, IGSF4 and SLIT2 is genes related to leukemia with promoter methylation ,the expression patterns of the 4 genes were detected with RT-PCR in HL60, U937 and MoM cell.
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为探讨SEMA6A、B56、IGSF4和SLIT2在人类白血病是否存在甲基化失活，本研究应用RT-PCR检测了这4个基因在HL60、U937和Molt4等3种常见的白血病细胞系的表达情况； |
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To study T cells' recognition, activation and frequency distribution is the most important component element in modern immunology.
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T细胞是机体免疫系统最关键的细胞之一，研究T细胞的识别、活化及频数分布是现代免疫学基础研究及应用研究的一个最重要的环节。 |
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Together with the advanced analysis method of LC/MS/MS, automatic sampling technique can dramatically improve the quality of pre-clinical research to meet the demand of high throughput drug screening.
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若能把自动生物样品采集技术和现代先进的LC/MS/MS分析方法合并使用,则可显著提高临床前研究的速度和质量,以满足高通量药物筛选的要求。 |
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Total DNA Extraction from Four Limestone Plants
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4种石山植物DNA提取方法筛选 |
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Total DNA extraction from Pericarpium Citri Reticulatae
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陈皮总DNA提取方法的研究 |
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Total Hepatic Irradiation by Cobalt - 60 Moving Strip Tech nique Short Term Result of 14 Cases of Advanced Primary Liver Cancer
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~(60)钴移动条技术全肝照射晚期原发性肝癌14例报告 |
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Total Iron and Protein Contents of Six Sorts of Animal Blood
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六种动物血的铁和蛋白质含量 |
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Total RNA was extracted from the human Molt-4 cells as template according to the instructions and then human CD81 gene was was amplified by RT-PCR and PCR. The PCR products was determined by Agarose gel electrophoresis.
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根据已公布的人CDS IcDNA序列(核酸库中的编号:NM-004356)，设计一对引物，然后取传代后的人Molt一4细胞，按照产品操作说明书从人Molt一4细胞提取总RNA，运用RT一PcR及PcR方法扩增出人CD81基因，PCR产物经l%琼脂糖凝胶电泳鉴定大小正确。 |
