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The tumor cell vaccine (TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL-2,TNF-α and GM-CSF).
中文意思:
方法以丝裂霉素C(MMC)处理高表达转m4-1BBL基因的小鼠Hepa1-6肝癌细胞,制成肿瘤细胞瘤苗(TCV),体外与同系小鼠脾淋巴细胞共同培养后,观察其对脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的影响。


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The technology of high cell density culture for this recombinant was established. In repeated culture for three times,the average cell density A 600 nm was 58,dry cell weight was 18.0 g/L and rIL 2 expression was 42.4% of total cell protein. 通过三批重复实验,确定了rIL-2工程菌高密度培养方案,平均菌密度A600nm值为58,菌干重为18.0g/L,rIL-2表达占菌体总蛋白的42.4%。
The temperature for Streptomyces albulus to grow was 28 C,the yield of glucose to Streptomyces albulus was 0.429 g. cell/g. sub, the field of Streptomyces albulus was 10.07g/L after the batch fermentation, the field of 拢 -PL was only 0.79g/L. 28℃时对细胞生长非常有利,菌体得率Y_(x/s)达到0.429g.cell/g.sub,发酵结束时菌体量最高为10.07g/L,但ε-聚赖氨酸产量仅0.79/L。
The therapeutic effects of Rituximab on 32 diffuse large B cell non - Hodgkin lymphoma cases 美罗华治疗32例弥漫型大B细胞性非何杰金氏淋巴瘤
The treatment of acute liver failure by auxiliary partial orthotopic liver transplantation in rats 大鼠原位辅助性肝移植治疗急性肝功能衰竭
The treatment of rat fulminant hepatic failure by auxiliary partial heterotopic liver transplantation:experimental study 辅助性肝移植治疗大鼠急性肝功能衰竭的实验研究
The tumor cell vaccine (TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC). Cocultivation TCV with syngeneic murine spleen cells and the supernatants were harvested for detecting the cytokines (IL-2,TNF-α and GM-CSF). 方法以丝裂霉素C(MMC)处理高表达转m4-1BBL基因的小鼠Hepa1-6肝癌细胞,制成肿瘤细胞瘤苗(TCV),体外与同系小鼠脾淋巴细胞共同培养后,观察其对脾细胞产生细胞因子(IL-2、TNF-α和GM-CSF)的影响。
The tumor specific CTL was generated form activated autologous T cell by DC vaccine loaded with tumor antigen, and the killing activity of CTL to CT26 cells was tested by the lactate dehydrogenase (LDH) release assay in vitro. 负载肿瘤抗原的DC疫苗激活同源T淋巴细胞产生细胞毒性T淋巴细胞(CTL),采用乳酸脱氢酶(LDH)4h释放法检测CTL在体外对CT26细胞的杀伤活性;
The tumors were predominate B cell lymphoma (5/7 cases)and less common in T cell(1/7 cases)and histiocyte lymphoma(1/7 cases). 多数为B细胞性淋巴瘤 ( 5 7例 ) ,T细胞性淋巴瘤 ( 1 7例 )和组织细胞性淋巴瘤少见 ( 1 7例 )。
The tumorswere predominate B cells lymphere(5/7 cases), occasional T cell 1/7 cases) and histiocyte lymphoma (1/7 cases). 多数为B细胞性淋巴瘤(5/7例),T细胞性淋巴瘤(1/7例)和组织细胞性淋巴瘤(1/7例)少见。
The ultrastructure of human mesenchymal stem cells 人骨髓间充质干细胞的超微结构
The use of joint imaging with 99m Tc labeled human immunoglobulin G in rheumatoid arthritis ~(99m)Tc标记人免疫球蛋白关节显像在类风湿关节炎中的应用
 
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