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Methods The following ways were used: Culturing neuroblastoma cell line Na in vitro and modeling ischemia reperfusion(firstly deprive of nutrition and oxygen for 90 minute,then reperfusion different times).
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方法 采用体外培养神经母细胞瘤细胞株Na,模拟缺血再灌注 (先缺氧缺营养 90min ,然后正常培养不同时间 ) ; |
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Methods The fragment of hsIL R gene obtained by PCR was inserted into clonal vector pALTER and sequenced.
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方法首先利用PCR技术扩增出人可溶性IL－R（hsIL－R）编码基因片段，并重组入克隆载体pALTER－。 |
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Methods The left pump delivered blood from left atrium to aorta and the right pump from right atrium to pulmonary main arteria.
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方法应用叶轮血泵行双心室辅助，左泵从左心房输送血液至主动脉，右泵从右心房输送血液至肺动脉。 |
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Methods The length from nostril to cardia(N CI),from nostril to pylorus(N PY)and from jugular notch to the joint of body of sternum and xiphoid process(J P)were measured on 0 adult cadaveric specimens(male , female ).
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方法 选 0例成人尸体 (男性 例 ,女性 例 )测量鼻孔至胃贲门的长度 (N CI)、鼻孔至胃幽门的长度 (N PY)及胸骨颈静脉切迹至剑胸结合处的长度 (J P) ,分别以N CI和N PY的长度为Y值 ,以J P的长度为X值 ,求回归方程。 |
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Methods The levels of mRNA for five kinesin superfamily genes,KIFA,KIF,KIF A,KIF, and KIFA,in cerebral cortex of adult and newborn SD rats,were estimated by RT? PCR.
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方法 运用RT PCR方法检测KIFA、KIF、KIF A、KIF与KIFA基因在新生 d大鼠和成年大鼠大脑皮质的表达。 |
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Methods The methods of fleetness investigation,informal discussion and statist ical analyses were used.
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方法 快速调 查、现场座谈和统计分析。 |
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Methods The smears of oral exfoliative cells were made from buccal mucosa,the dorsum and ventrum of the tongue from 90 normal individuals.
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方法 对90例正常口腔粘膜颊部、舌背、舌腹 个部位进行了脱落细胞微核计数的分析 ,并判定微核计数的影响因素。 |
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Methods The splitting was performed in %sucrose, splitting plus (SP, pullman, WA USA), and mPBS respectively using a glass needle in hand.
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方法 在mPBS、 %蔗糖液和进口分割液三种不同分割液中对桑椹胚和囊胚进行徒手分割。 |
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Methods The therapeutic effect of collateral branch root canal filled with hot gutta percha guillotine operation on periapical lesions was observed and the related clinical cases were analysed.
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方法 通过临床病例分析 ,观察热牙胶挤压术充填侧支根管对根尖周病变的治疗效果。 |
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Methods The thickness and the transverse diameter of L ～S inter vertebral disc and the vertical diameter of the bone hole were measured on sides of adult spine (including pelves).
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方法 用 例成人脊柱 (包括骨盆 )标本 ,对L～S间盘厚度、横径以及骨孔的上下径进行了观测。 |
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Methods The time, inducement, anterior chamber exudate and cortex lentis residue of cases were observed.
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方法 对 例病人的发病时间、诱因、前房渗出和皮质残留情况进行观察。 |
