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Chorismate mutase (CM ), prephenate dehydratase (PD ), and phenylalanine aminotransferase (PAT) are the three key enzymes in the fermentation of phenylalanine. In E. colt,the first two are encoded by a single gene pheA,while the third by tyrB gene.
中文意思:
为探讨用基因工程的手段改良苯丙氨酸的发酵菌株,采用聚合酶链反应(PCR)的方法,从大肠杆菌总DNA中克隆得到了编码苯丙氨酸合成途中的两个关键酶基因--即分枝酸变位酶(CM)/预苯酸脱水酶(PD)基因pheA与苯丙氨酸转氨酶(PAT)基因tyrB,在大肠杆菌中进行了这两个基因的单个和串联表达。


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Chorismate mutase (CM ), prephenate dehydratase (PD ), and phenylalanine aminotransferase (PAT) are the three key enzymes in the fermentation of phenylalanine. In E. colt,the first two are encoded by a single gene pheA,while the third by tyrB gene.为探讨用基因工程的手段改良苯丙氨酸的发酵菌株,采用聚合酶链反应(PCR)的方法,从大肠杆菌总DNA中克隆得到了编码苯丙氨酸合成途中的两个关键酶基因--即分枝酸变位酶(CM)/预苯酸脱水酶(PD)基因pheA与苯丙氨酸转氨酶(PAT)基因tyrB,在大肠杆菌中进行了这两个基因的单个和串联表达。
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