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Methods The MK levels in serum were determined with enzyme-linked immunosorbent assays for 20 healthy women,20 patients with breast benign diseases and 64 patients with breast cancer.
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方法采用酶联免疫吸附法对20例正常女性,20例女性乳腺良性疾病和64例乳腺癌患者血清MK进行检测。 |
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Methods The TK6 cell was cultured and its genomic DNA was extracted and prepared to the single-stranded probes of the exon 2 of k-ras gene.
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方法培养TK6细胞,抽提基因组DNA,制备k-ras基因外显子2的单链探针。 |
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Methods The allergen of inhalation and edibility in 311 cases with allergic dermatitis and 30 healthy persons were detected by enzyme-linked immunosorbent assay (ELISA).
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方法采用酶联免疫吸附试验(ELISA)对311例过敏性皮肤病患者和30名健康对照者血清中吸入性及食入性过敏原进行检测。 |
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Methods The effects of dopamine (DA) and glutathione (GSH) on apoptotic PC12 cell were examined by fluorescence microscopy using the DNA end- labeling method.
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方法用脱氧核糖核酸（DNA）标记法，借助荧光显微镜观察多巴胺（DA）和还原型谷胱甘肽（GSH）对PC12细胞凋亡的影响。 |
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Methods The expression levels of the P53 protein and Ki-67 were examined by immunohistochemistry(S-P method). To analysis the relationship between levels of P53 protein and Ki-67 and differentiation,clinical stage and metastasis of esophageal carcinoma.
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方法应用免疫化学S-P法对50例食管鳞状细胞癌中P53蛋白和Ki-67的表达水平进行研究,分析其与食管癌组织分化程度、临床分期及转移的关系。 |
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Methods The heparinaseⅡ gene was amplified with PCR from the genomic DNA of Flavobacterium heparinum, and it was linked into expression plasmid pET-28a after sequencing, then the recombined plasmid was expressed in E.
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方法通过PCR从黄杆菌基因组DNA中扩增出黄杆菌HepⅡ的基因,经验证后插入到表达质粒pET-28a上构建表达载体,重组质粒转化E. |
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Methods The level of Th1 and Th2 cytokines in serum were detected by flow fluorenscence immunmicrobeads assay (FFIA) from 44 patients with breast cancer and 47 cases of patients with breast benign Tumour.
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方法用流式荧光免疫微球分析技术(Flow Fluorenscence Immunmicrobeads Assay,FFIA)检测乳腺良、恶性肿瘤患者血清Th1/Th2细胞因子水平。 |
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Methods The samples of serum and BALF were obtained from 47 patients with untreated primary lung cancer and 18 patients with benign pulmonary diseases. The levels of endostatin were analyzed by enzyme-linked immunosorbent assay(ELISA).
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方法采用酶联免疫吸附法(En-zyme-linked immunosorbent assay,ELISA)检测初诊肺癌47例及肺良性病变18例患者外周血清及BALF中Endostatin的表达水平。 |
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Methods The serum anti-β_2GPI and anti-cardiolipin antibodies(ACA) were measured by enzyme-linked immunosorbent assay(ELISA) in 56 patients with SLE and 40 healthy controls.
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方法采用酶联免疫吸附试验(ELISA)半定量测定56例SLE患者及40名健康对照者血清抗β2GPI和抗心磷脂抗体(ACA)阳性率。 |
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Methods The serum concentrations of AOPP and AGE were measured by using spectrophotometer and enzyme linked immunosorbent assay in 29 patients with NSTEMI/UA and 26 patients with STEMI (ACS group) and 30 healthy subjects (control group).
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方法选择ACS患者共55例,其中非ST段抬高ACS(NSTEMI/UA)组29例,ST段抬高ACS(STEMI)组26例,正常对照组30例作为研究对象,采用分光光度计法检测所有入选者的血清AOPP浓度,抗戚蓝蛋白晚期糖基化终末产物抗体竞争酶联免疫吸附测定法测定血清AGE水平,并记录4周内所有患者的病情转归情况。 |
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Methods The serum levels of MPO were determined by enzyme-linked immunosorbent assay(ELISA) in 67 patients diagnosed as coronary artery atherosclerosis by angiography and 20 cases which angiography was normal as control group.
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方法应用酶联免疫吸附试验(ELISA)测定67例经冠状动脉造影证实的冠心病患者和20例造影阴性对照者的MPO水平,冠心病患者按造影结果分为3组。 |
