以下为句子列表：
英文: For inoculation of an agar deep tube, a straight needle is inserted to the bottom of the tube in a straight line and rapidly withdrawn along the line of insertion.
中文: 在接种试管深层培养基时，一支直的接菌针直线地穿刺到试管的底部，同时快速地沿著接种线抽出。
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英文: Methods The improvements of the operation included; a supertemporal conjunctival flap 180° with its base at the fornix, the puncture points were located at 8 and 2 o'clock (right eye) or 10 and 4 o'clock (left eye), the straight needle and shovel needle w
中文: 方法术式改进包括：颞上方＞180°、以穹隆为基底的大结膜瓣；穿刺点选择8点及2点位（右眼）或10及4点位（左眼）；用聚丙烯缝线所带直针及铲针，从角膜切口两端进入眼内，由8点及2点位巩膜瓣下穿刺出针。
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