以下为句子列表：
英文: In this paper, three major biotechnologies including tissue culture, gene engineering and molecular markers were explained. And its development direction in the future was discussed.
中文: 摘要阐述了组织培养、基因工程技术和分子标记技术在花椰菜育种中的应用现状，对今后生物技术在花椰菜育种中应用的发展方向进行了讨论。
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