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srna

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英文: A proteinase K digestion and phenol-chloroform extraction method was used to extract IBDV dsRNA from bursae of infected chicken. The VP gene was amplified by RT-PCR, and then cloned into pMD8-T vector for sequence analysis.

中文: 用蛋白酶K消化、酚-氯仿抽提法成功提取出IBDV基因组RNA,用RT-PCR技术扩增出IBDV的结构蛋白基因VP,并将其克隆到pMD8-T载体。        更详细...
英文: Typical rotavirus particles were seen with direct electron microscope. Polyacrylamide gel electrophoresis (PAGE) of its dsRNA genome showed its RNA electropherotype was 4:2:3:2 which accords with the electropherotype of guoup A rotavirus.

中文: 病毒核酸的聚丙烯酰胺凝胶电泳(PAGE)表明:其电泳型为4:2:3:2型,属于A群轮状病毒。        更详细...
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